YdgT, the Hha paralogue in Escherichia coli, forms heteromeric complexes with H-NS and StpA
نویسندگان
چکیده
منابع مشابه
Functions of the Hha and YdgT Proteins in Transcriptional Silencing by the Nucleoid Proteins, H-NS and StpA, in Escherichia coli
The Hha and YdgT proteins are suggested to modulate the expression of horizontally acquired genes by interacting with H-NS and StpA, which play central roles in the transcriptional silencing of such genes. However, it is also possible that Hha/YdgT repress gene expression independently of H-NS/StpA, as we have not fully understood the molecular mechanism through which Hha/YdgT modulate H-NS/Stp...
متن کاملHeteromeric interactions among nucleoid-associated bacterial proteins: localization of StpA-stabilizing regions in H-NS of Escherichia coli.
The nucleoid-associated proteins H-NS and StpA in Escherichia coli bind DNA as oligomers and are implicated in gene regulatory systems. There is evidence for both homomeric and heteromeric H-NS-StpA complexes. The two proteins show differential turnover, and StpA was previously found to be subject to protease-mediated degradation by the Lon protease. We investigated which regions of the H-NS pr...
متن کاملHha has a defined regulatory role that is not dependent upon H-NS or StpA
The Hha family of proteins is involved in the regulation of gene expression in enterobacteria by forming complexes with H-NS-like proteins. Whereas several amino acid residues of both proteins participate in the interaction, some of them play a key role. Residue D48 of Hha protein is essential for the interaction with H-NS, thus the D48N substitution in Hha protein abrogates H-NS/Hha interactio...
متن کاملGene silencing H-NS paralogue StpA forms a rigid protein filament along DNA that blocks DNA accessibility
Nucleoid-associated proteins are bacterial proteins that are responsible for chromosomal DNA compaction and global gene regulation. One such protein is Escherichia coli Histone-like nucleoid structuring protein (H-NS) which functions as a global gene silencer. Whereas the DNA-binding mechanism of H-NS is well-characterized, its paralogue, StpA which is also able to silence genes is less underst...
متن کاملNucleotide sequence of a newly-identified Escherichia coli gene, stpA, encoding an H-NS-like protein.
analysis of a 978-bp fragment of this segment revealed an ORF , w . r o , * r. A A , ^ c ,,noo> ^ * o i«> M.-> , J , r . b r IIA • J /i-.N 1Martinez,E., Bartolomi.B. and de la Cruz,F. (1988) Gene 68, 159-162. that encodes a putative protein of 134 ammo acids (Figure 1). 2 DevereuxJ., Haeberli.P. and Smithies.O. (1984) Nucleic Acids Res. 12, Comparative searches (FASTA, TFASTA (2)) showed that t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 2004
ISSN: 0950-382X,1365-2958
DOI: 10.1111/j.1365-2958.2004.04268.x